Supplemental Fig. S1. Phylogenetic tree of the family Betulaceae based on nucleotide sequences of the nuclear ITS regions, depicted by RAxML-NG version 1.2.0. The evolutionary history was inferred by using the maximum likelihood (ML) method based on the JC model. Accession numbers were listed behind each name. Statistical support values more than 50 % obtained by RAxML-NG for bootstrap probabilities (BP) with 10,000 replicates are shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The root is arbitrarily placed on the branch leading to the clade which includes species of the Ticodendron incognitum and Myrica gale var. tomentosa, following Yang et al. (2021) and Stevens (2023).
Supplemental Fig. S2. Phylogenetic tree of the family Caprifoliaceae based on nucleotide sequences of the chloroplast atpB-rbcL region, depicted by RAxML-NG version 1.2.0. The evolutionary history was inferred by using the maximum likelihood (ML) method based on the TVM + I + Γ model. Accession numbers were listed behind each name. Statistical support values more than 50 % obtained by RAxML-NG for bootstrap probabilities (BP) with 10,000 replicates are shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The root is arbitrarily placed on the branch leading to the clade which includes species of the subfamily Diervilliodeae, following Lee et al. (2021) and Wang et al. (2020).
Appendix 5. Supplementary data 1: the relative abundance with log-transformation of OTUs between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).
Appendix 6. Supplementary data 2: all predicted bacterial metabolic pathways between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).
Appendix 7. Supplementary data 3: the two predicted potential degradation of allelopathic compounds pathway, as well as associated bacterial groups between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).
Fig. S1. Phylogenetic tree of the family Amaranthaceae based on the nuclear ribosomal internal transcribed spacer (ITS) region, depicted by RAxML-NG version 0.9.0git. The evolutionary history was inferred by using the maximum likelihood method based on the SYM + I + G model. Accession numbers were listed behind each name. Statistical support values more than 50 % obtained by RAxML-NG for bootstrap probabilities (BP) with 10,000 replicates and BEAST for Bayesian posterior probabilities (PP) with 10,000,000 replicates are shown on or near nodes (BP/PP). The tree was rooted at the midpoint. The pdf of Supplementary Figure S1 can be downloadable via the web link https://www.digital-museum.hiroshima-u.ac.jp/~main/index.php/Supplementary_data.
Fig. S2. Phylogenetic tree of the family Amaranthaceae based on the chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit (rbcL) gene sequence, depicted by RAxML-NG version 0.9.0git. The evolutionary history was inferred by using the maximum likelihood method based on the TVM + I + G model. Accession numbers were listed behind each name. Statistical support values more than 50 % obtained by RAxML-NG for bootstrap probabilities (BP) with 10,000 replicates and BEAST for Bayesian posterior probabilities (PP) with 10,000,000 replicates are shown on or near nodes (BP/PP). The tree was rooted at the midpoint. The pdf of Supplementary Figure S2 can be downloadable via the web link https://www.digital-museum.hiroshima-u.ac.jp/~main/index.php/Supplementary_data.