Sheng Wasaki Tsubota 2022

Sheng et al. (2022)

• Sheng, Z.-P., Wasaki, J. & Tsubota, H. 2022. A preliminary study on the influence of invasive plant through allelopathy: effects of Chinese tallow tree, Triadica sebifera (Euphorbiaceae), on its rhizosphere microbial community in Miyajima Island, SW Japan. Hikobia 18: 199-220.

Abstract

Invasive plants can release allelopathic compounds that inhibit or enhance the growth of other plants and microorganisms in the surrounding environment. In this study, we performed the next-generation sequencing (NGS) analyses to compare the genes of the soil microbial communities in a natural forest and an open place where Triadica sebifera (L.) Small, a typical invasive plant, grows in Miyajima Island, Hiroshima Prefecture, Japan. We collected soil samples in May 2022, the litter layer of Chinese tallow completely decomposed. Our data suggested that the invaded site of T. sebifera have different genetic composition of soil bacterial and fungal species, and in bacterial metabolic pathways. The result also seemed indicate that T. sebifera recruited several microbes including plant growth-promoting rhizobacteria (PGPRs), such as Sphingomonas, which may be involved two potential degradations of allelopathic compounds in the rhizosphere soil of Chinese tallow tree. A more detailed chemical analysis will also be needed to distinguish whether the allelopathic substances recruiting microorganisms come from the fallen leaves or from the exudation of roots. This study provided a new insight into the invasion and establishment of invasive plants with the possibility of bacteria involved allelopathy process in the soil. We also found that using different kits for extracting DNA and PCR could affect the NGS results.

ノート

• ナンキンハゼの根圏のバクテリア相について次世代シーケンサーのデータにもとづいて報告した．

インターネットリソース

DOI

• https://doi.org/10.60287/hikobia.18.4_199

Supplementary data

The files of Supplementary Data 1-3 of Sheng et al. (2022)

• Sheng, Z.-P., Wasaki, J. & Tsubota, H. 2022. A preliminary study on the influence of invasive plant through allelopathy: effects of Chinese tallow tree, Triadica sebifera (Euphorbiaceae), on its rhizosphere microbial community in Miyajima Island, SW Japan. Hikobia 18: 199-220.
  • Supplementary data. The files of Supplementary Data 1-3 can be downloadable via the web link https://www.digital-museum.hiroshima-u.ac.jp/~main/index.php/Supplementary_data.
    • ファイル:Appendix5 Supplementary data 1 Sheng et al 2022 Hikobia.xls
      • Appendix 5. Supplementary data 1: the relative abundance with log-transformation of OTUs between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).
    • ファイル:Appendix6 Supplementary data 2 Sheng et al 2022 Hikobia.xls
      • Appendix 6. Supplementary data 2: all predicted bacterial metabolic pathways between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).
    • ファイル:Appendix7 Supplementary data 3 Sheng et al 2022 Hikobia.xls
      • Appendix 7. Supplementary data 3: the two predicted potential degradation of allelopathic compounds pathway, as well as associated bacterial groups between M (N = 3) and S (N = 3) groups. M responds to the native site (Murohama); S responds to the invasive site (Shobunjo).

Supplementary figures

• https://www.digital-museum.hiroshima-u.ac.jp/~main/index.php/Supplementary_data
• Supplementary_data