PCR primers

chloroplast

rps4 (ribosomal protein S4; ribosomal protein subunit 4)

Table. Primers used for PCR amplification and sequencing
Analyzed region	Forward / Reverse	Primer name	Sequence (5'–3')	References	For
rps4	Forward	trnT36R	GTAATGCGAT GGTCATCGGT TCGACTCCGA TA	Inoue et al. (2012)	Bryophytes
rps4	Forward	rps5	ATGTCCCGTT ATCGAGGACC T	Nadot et al. (1994)	Bryophytes
rps4	Forward	rps4_1R	ATGTCCCGTT ATCGAGGACC TCGTGTA	Inoue et al. (2012)	Bryophytes
rps4	Forward	rps4_2Fmas	TGTCCCGTTA TCGAGGACC	Masuzaki et al. (2010)	Bryophytes
rps4	Forward	rps4_19Fi	CCTCGTGTAA GAATAATACG TC	Inoue & Tsubota (2014)	Bryophytes
rps4	Forward	rps4_275Fmas	AGATAATGTG ATCTTTCGAT TAGGTATGGC	Masuzaki et al. (2010)	Bryophytes
rps4	Forward	rps4_347Fmas	CAATTGGTTA ATCATAGACA	Masuzaki et al. (2010)	Bryophytes
rps4	Forward	rps4_553Fmas	CAAACGATTA ATCGTGAATG GA	Masuzaki et al. (2010)	Bryophytes

rps4	Reverse	psaA340F	CTTGAGCACT AGGTTTAATA TGAGTAGGAT CA	Inoue et al. (2012)	Bryophytes
rps4	Reverse	trnS (syn. trans, trnS-F)	TACCGAGGGT TCGAATC	Souza-Chies et al. (1997), Buck et al. (2000)	Vascular plants (Souza-Chies et al. 1997), Bryophytes (Buck et al. 2000, as trans)
rps4	Reverse	rps4_609RL	TTAAGCTTGA CGAGAATAAT ATTC	Masuzaki et al. (2010)	Bryophytes
rps4	Reverse	psa3'	ATATTCTACA ACTAACAACT C	Nadot et al. (1994)	Bryophytes
rps4	Reverse	rps4_602Fn (~~psa4_602Fn~~)	TGACGAGAAT AATATTCTAC AACTA	Inoue & Tsubota (2014)	Bryophytes
rps4	Reverse	rps4_605Rmas	ACTTGGCGAG AATAATATTC AAC	Masuzaki et al. (2010)	Bryophytes
rps4	Reverse	rps4_578Ri	CGAGAATAAT ATTCTACAAC TA	Inoue & Tsubota (2014)	Bryophytes
rps4	Reverse	rps4_531Rmas	TCCATTCACG ATTAATCGTT TG	Masuzaki et al. (2010)	Bryophytes
rps4	Reverse	rps4_305Rmas	GCCATACCTA ATCGAAAGAT CACATTATCT	Masuzaki et al. (2010)	Bryophytes
rps4	Reverse	rps4-166F	CCATAATGAA AACGTAATTT TTG	Hernández-Maqueda et al. (2008), Wynns & Lange (2014)	Bryophytes

rps4–trnT–trnL–trnF

Table. Primers used for PCR amplification and sequencing
Analyzed region	Forward / Reverse	Primer name	Sequence (5'–3')	References	For
rps4–trnT–trnL–trnF	Forward	rps4_609RL	TTAAGCTTGA CGAGAATAAT ATTC	Masuzaki et al. (2010)	Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnT–trnL–trnF	Forward	trnT71F	CCGCTATCGG AGTCGAACCG ATGACCATCG	Tsubota et al. (2015b)	Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF	Forward	a [trnT(UGU)F-a]	CATTACAAAT GCGATGCTCT	Taberlet et al. (1991)	Vascular plants
trnL–trnF	Forward	c [trnL(UAA)5exonF-c]	CGAAATCGGT AGACGCTACG	Taberlet et al. (1991)	Vascular plants
trnL–trnF	Forward	g	GGGCAATCCT GAGCCAA	Taberlet et al. (2007)	Vascular plants
trnL–trnF	Forward	e [trnL(UAA)3exonF-e]	GGTTCAAGTC CCTCTATCCC	Taberlet et al. (1991)	Vascular plants

rps4–trnT–trnL–trnF	Reverse	trnF1F	TGCCAGAAAC CAGATTTGAA CTGGTGACAC	Tsubota et al. (2015b)	Vascular plants (Lysimachia, Tsubota et al. 2015b)
rps4–trnT–trnL–trnF	Reverse	trnF8F	TCAGTCCTCT GCTCTACCAA CTGAGCTA	Tsubota et al. (2015b)	Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF	Reverse	f [trnF(GAA)R-f]	ATTTGAACTG GTGACACGAG	Taberlet et al. (1991)	Vascular plants
trnL–trnF	Reverse	d [trnL(UAA)3exonR-d]	GGGGATAGAG GGACTTGAAC	Taberlet et al. (1991)	Vascular plants
trnL–trnF	Reverse	h	CCATTGAGTC TCTGCACCTA TC	Taberlet et al. (2007)	Vascular plants
trnL–trnF	Reverse	b [trnL(UAA)5exonR-b]	TCTACCGATT TCGCCATATC	Taberlet et al. (1991)	Vascular plants

Example of primer pairs

trnF1F & trnS (for rps4-trnT-trnL-trnF)
trnF1F & trnT71F (for trnL-trnF region)
trnT36R & trnS (for rps4 gene)

References

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PCR primers cp rps4-trnT-trnL-trnF

目次

PCR primers

chloroplast

rps4 (ribosomal protein S4; ribosomal protein subunit 4)

rps4–trnT–trnL–trnF

Example of primer pairs

References

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