PCR primers cp rps4-trnT-trnL-trnF
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PCR primers
chloroplast
rps4 (ribosomal protein S4; ribosomal protein subunit 4)
Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
rps4 | Forward | trnT36R | GTAATGCGAT GGTCATCGGT TCGACTCCGA TA | Inoue et al. (2012) | Bryophytes |
rps4 | Forward | rps5 | ATGTCCCGTT ATCGAGGACC T | Nadot et al. (1994) | Bryophytes |
rps4 | Forward | rps4_1R | ATGTCCCGTT ATCGAGGACC TCGTGTA | Inoue et al. (2012) | Bryophytes |
rps4 | Forward | rps4_2Fmas | TGTCCCGTTA TCGAGGACC | Masuzaki et al. (2010) | Bryophytes |
rps4 | Forward | rps4_19Fi | CCTCGTGTAA GAATAATACG TC | Inoue & Tsubota (2014) | Bryophytes |
rps4 | Forward | rps4_275Fmas | AGATAATGTG ATCTTTCGAT TAGGTATGGC | Masuzaki et al. (2010) | Bryophytes |
rps4 | Forward | rps4_347Fmas | CAATTGGTTA ATCATAGACA | Masuzaki et al. (2010) | Bryophytes |
rps4 | Forward | rps4_553Fmas | CAAACGATTA ATCGTGAATG GA | Masuzaki et al. (2010) | Bryophytes |
rps4 | Reverse | psaA340F | CTTGAGCACT AGGTTTAATA TGAGTAGGAT CA | Inoue et al. (2012) | Bryophytes |
rps4 | Reverse | trnS (syn. trans, trnS-F) | TACCGAGGGT TCGAATC | Souza-Chies et al. (1997), Buck et al. (2000) | Vascular plants (Souza-Chies et al. 1997), Bryophytes (Buck et al. 2000, as trans) |
rps4 | Reverse | rps4_609RL | TTAAGCTTGA CGAGAATAAT ATTC | Masuzaki et al. (2010) | Bryophytes |
rps4 | Reverse | psa3' | ATATTCTACA ACTAACAACT C | Nadot et al. (1994) | Bryophytes |
rps4 | Reverse | rps4_602Fn ( |
TGACGAGAAT AATATTCTAC AACTA | Inoue & Tsubota (2014) | Bryophytes |
rps4 | Reverse | rps4_605Rmas | ACTTGGCGAG AATAATATTC AAC | Masuzaki et al. (2010) | Bryophytes |
rps4 | Reverse | rps4_578Ri | CGAGAATAAT ATTCTACAAC TA | Inoue & Tsubota (2014) | Bryophytes |
rps4 | Reverse | rps4_531Rmas | TCCATTCACG ATTAATCGTT TG | Masuzaki et al. (2010) | Bryophytes |
rps4 | Reverse | rps4_305Rmas | GCCATACCTA ATCGAAAGAT CACATTATCT | Masuzaki et al. (2010) | Bryophytes |
rps4 | Reverse | rps4-166F | CCATAATGAA AACGTAATTT TTG | Hernández-Maqueda et al. (2008), Wynns & Lange (2014) | Bryophytes |
rps4–trnT–trnL–trnF
Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
rps4–trnT–trnL–trnF | Forward | rps4_609RL | TTAAGCTTGA CGAGAATAAT ATTC | Masuzaki et al. (2010) | Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b) |
trnT–trnL–trnF | Forward | trnT71F | CCGCTATCGG AGTCGAACCG ATGACCATCG | Tsubota et al. (2015b) | Vascular plants (Lysimachia, Tsubota et al. 2015b) |
trnL–trnF | Forward | a [trnT(UGU)F-a] | CATTACAAAT GCGATGCTCT | Taberlet et al. (1991) | Vascular plants |
trnL–trnF | Forward | c [trnL(UAA)5exonF-c] | CGAAATCGGT AGACGCTACG | Taberlet et al. (1991) | Vascular plants |
trnL–trnF | Forward | g | GGGCAATCCT GAGCCAA | Taberlet et al. (2007) | Vascular plants |
trnL–trnF | Forward | e [trnL(UAA)3exonF-e] | GGTTCAAGTC CCTCTATCCC | Taberlet et al. (1991) | Vascular plants |
rps4–trnT–trnL–trnF | Reverse | trnF1F | TGCCAGAAAC CAGATTTGAA CTGGTGACAC | Tsubota et al. (2015b) | Vascular plants (Lysimachia, Tsubota et al. 2015b) |
rps4–trnT–trnL–trnF | Reverse | trnF8F | TCAGTCCTCT GCTCTACCAA CTGAGCTA | Tsubota et al. (2015b) | Vascular plants (Lysimachia, Tsubota et al. 2015b) |
trnL–trnF | Reverse | f [trnF(GAA)R-f] | ATTTGAACTG GTGACACGAG | Taberlet et al. (1991) | Vascular plants |
trnL–trnF | Reverse | d [trnL(UAA)3exonR-d] | GGGGATAGAG GGACTTGAAC | Taberlet et al. (1991) | Vascular plants |
trnL–trnF | Reverse | h | CCATTGAGTC TCTGCACCTA TC | Taberlet et al. (2007) | Vascular plants |
trnL–trnF | Reverse | b [trnL(UAA)5exonR-b] | TCTACCGATT TCGCCATATC | Taberlet et al. (1991) | Vascular plants |
Example of primer pairs
- trnF1F & trnS (for rps4-trnT-trnL-trnF)
- trnF1F & trnT71F (for trnL-trnF region)
- trnT36R & trnS (for rps4 gene)
References
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関連ページ
- UBC primers
- IUPAC/UBMB codes for nucleic acid bases [Nucleotide base codes (IUPAC)]
- Nucleotide base codes
- pyrimidine
- purine
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