PCR primers nr 18SrRNA ITSs

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PCR primers

nuclear

18S rRNA gene and ITS region

Table. Primers used for PCR amplification and sequencing of the nr 18S rRNA gene and ITS regions. The numbers within the primer name correspond to positions in the 18S rRNA gene of Chlorella vulgaris (X13688) and the 5.8S rRNA gene of Chlamydomonas tetragama (AB004866).
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
18S rRNA gene Forward 18SPrimer A CCGAATTCGT CGACAACCTG GTTGATCCTG CCAGT Medlin et al. (1988) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S1Fh AACCTGGTTG ATCCTGCC Nakahara et al. (2004a) Algae, Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Forward 18S2Fh GTTGATCCTG CCAGTAGTCA Nakahara et al. (2004a) Algae
18S rRNA gene Forward 18SNS1 GTAGTCATAT GCTTGTCTC White et al. (1990); Kocher & White (1992) Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S156 TCTAGAGCTA ATACGTGCG Nakahara et al. (2004b) Algae
18S rRNA gene Forward 18S325R CTGCCCTATC AACTTTCG Nakahara et al. (2004b) Algae (Chlorella etc.)
18S rRNA gene Forward 18S323 CGATGGTAGG ATAGAGG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S410 CCACATCCAA GGAAGGCAGC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Forward 18S575 CGGTAATTCC AGCTCCA Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S578 CCAGCTCCAA TAGCGTATAT TT Nakahara et al. (2004b) Algae
18S rRNA gene Forward 18S705 GGCTCCTGGG CTTCACTG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S791 AGCATGGGAT AACACGATAG GA Nakahara et al. (2004b) Algae
18S rRNA gene Forward 18S921 GAAAGACGAA CTACTGCGA Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Forward 18S1171 GCTTAATTTG ACTCAACAC Tsubota et al. (2012) Algae, Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S1208 TACCAGGTCC AGACATAGTG AGG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S1288 TGGGTTGCCT TGTCA Nakahara et al. (2004a) Algae
18S rRNA gene Forward 18S1307 ACGAGACCTC AGCCTGCT Nakahara et al. (2004a) Algae
18S rRNA gene Forward 18S1421 CAGGTCTGTG ATGCCCTTAG A Nakahara et al. (2004a) Algae, Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Forward 18S1560 GCGTTGATTA CGT Nakahara et al. (2004b) Algae
ITS Forward 18S1659B CGTCGCTCCT ACCGATTG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Forward 18S1764B AGAGGAAGGA GAAGTCGTAA C Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Forward 18SrDNAHTF1 AAGGTTTCCG TAGGTGAACC TGC Tsubota et al. (2012) Vascular plants (Lactuca, Sisymbrium etc.)
ITS Forward 18srRNA TCCGTAGGTG AACCTGCGG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.)
ITS Forward 5.8S10B CTCAGCAACG GATATCTTGG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Forward 5srRNA ACGATGAAGA ACGCAGCG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Forward 5.8S43H GAAGAACGTA GCGAAATGCG A Tsubota et al. (2009) Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Forward 5.8S51HV TAGCGAAATG CGATACTTGG Tsubota et al. (2013) Vascular plants (Sisymbrium etc.)
ITS Forward 5.8S114HV AGTTGCGCCC GAAGC Tsubota et al. (2013) Vascular plants (Sisymbrium etc.)
ITS Reverse 26S166BR GAGGACGCTT CTCCAGACTA C Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Reverse 26S102BR CCGGTTCGCT CGCCG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Reverse 25srRNA TCCTCCGCTT AGTGATATGC Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.)
ITS Reverse 26SrDNAHTR1 AAGGGAATCC TTGTAAGTTT CT Tsubota et al. (2012) Vascular plants (Lactuca, Sisymbrium etc.)
ITS Reverse 26SrDNAHTR2 AAGTTTCTTT TCCTCCGCTT A Tsubota et al. (2012) Vascular plants (Lactuca, Sisymbrium etc.)
ITS Reverse 5.8S124HR CGGGCGCAAC TTGCGTTCA Tsuota et al. (2009) Vascular plants (Halophila, Lactuca, Sisymbrium etc.)
ITS Reverse 5.8S102BR ACTCGATGAT TCGCGGA Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
18S rRNA gene Reverse 5.8SR–2 ACTACGTATC GCATTTCGCT Nakahara et al. (2004b) Algae
18S rRNA gene Reverse 5.8SR–3 CATTTCGCTG CGTTCTTCAT Nakahara et al. (2004b) Algae
18S rRNA gene & ITS Reverse 5.8SR-1 GTATCGCATT TCGCTGCGTT CTTCATCG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene & ITS Reverse 5srRNAR CGCTGCGTTC TTCATCGT Handa et al. (2003); Oguri et al. (2003) Algae (Stichococcus etc.), Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.)
18S rRNA gene Reverse 18Sprimer B CCCGGGATCC AAGCTTGATC CTTCTGCAGG TTCACCTAC Medlin et al. (1988) Algae (Stichococcus etc.)
18S rRNA gene Reverse 18S3Rh TGATCCTTCT GCAGGTTC Nakahara et al. (2004a) Algae, Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Reverse 18S4Rh CTGCAGGTTC ACCTACGGA Nakahara et al. (2004a) Algae
18S rRNA gene Reverse 18S1747R CTCCTTCCTC TAGGTGGGA Nakahara et al. (2004b) Algae
18S rRNA gene Reverse 18S1617R CAGGGACGTA ATCAACGC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18S1442R TCTAAGGGCA TCACAGAC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Reverse 18S1317R TTACCGGAAT CAACCTGACA AGGCAAC Nakahara et al. (2004b) Algae
18S rRNA gene Reverse 18S1125R CAGCCTTGCG ACCATACTCC Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Reverse 18S909R CCAAGAATTT CACCTCTGAC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Reverse 18SNS2 GGCTGCTGGC ACCAGACTTG C White et al. (1990); Kocher & White (1992) Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.)
18S rRNA gene Reverse 18S325R++[1][2] CGAAAGTTGA TAGGGCAG Nakahara et al. (2004b); Tsubota et al. (2012) Algae, Vascular plants (Lactuca etc.)

脚注

  1. 坪田ほか(2012)では表中で初出となっているが,実際にはNakahara et al.(2004b)が初出.
  2. The first appearance of this primer was not in Table 1 of Tsubota et al. (2012) but in Table 2 of Nakahara et al. (2004b).

References


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