PCR primers cp psbA-trnH

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PCR primers

chloroplast

psbA-trnH or trnH-psbA [trnH(GUG)-psbA intergenic region; trnH/psbA spacer; chloroplast photosystem II protein D1 (psbA) gene, partial cds; psbA-trnH intergenic spacer, complete sequence; and tRNA-His (trnH) gene, partial sequence]

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
psbA-trnH Forward psbAFh5s GTTATGCATG AACGTAA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Forward psbAF (as Reverse primer named psbA-R in Newmaster & Ragupathy 2009) GTTATGCATG AACGTAATGC TC Sang et al. (1997), Newmaster & Ragupathy (2009) Vascular plants (Sang et al. 1997, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Forward (as Reverse in Hamilton 1999) psb_A CGAAGCTCCA TCTACAAATG G Hamilton (1999) Vascular plants (Hamilton 1999)
psbA-trnH Reverse trnHRh6s CGCGCATGGT GGATTCA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Reverse trnHR CGCGCATGGT GGATTCACAA ATC Sang et al. (1997) Vascular plants (Sang et al. 1997)
psbA-trnH Reverse trnHf_05 (as Forward primer named trnH-F in Newmaster & Ragupathy 2009) CGCGCATGGT GGATTCACAA TCC Tate & Simpson 2003, Newmaster & Ragupathy (2009) Vascular plants (Tarasa by Tate & Simpson 2003, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Reverse (as Forward in Hamilton 1999) trn_H_(GUG) ACTGCCTTGA TCCACTTGGC Hamilton (1999) Vascular plants (Hamilton 1999)

References


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