PCR primers

提供: 広島大学デジタル自然史博物館 植物
移動: 案内検索

広島大学 > デジタル自然史博物館 > 植物 > コケ植物(蘚苔類) > 情報

目次

PCR primers

chloroplast

nuclear


PCR primers

chloroplast

rbcL (atpB-rbcL-trnR-zfpA)

rps4

rps4trnTtrnLtrnF

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4trnTtrnLtrnF Forward rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnTtrnLtrnF Forward trnT71F CCGCTATCGG AGTCGAACCG ATGACCATCG Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnLtrnF Forward a [trnT(UGU)F-a] CATTACAAAT GCGATGCTCT Taberlet et al. (1991) Vascular plants
trnLtrnF Forward c [trnL(UAA)5exonF-c] CGAAATCGGT AGACGCTACG Taberlet et al. (1991) Vascular plants
trnLtrnF Forward e [trnL(UAA)3exonF-e] GGTTCAAGTC CCTCTATCCC Taberlet et al. (1991) Vascular plants
rps4trnTtrnLtrnF Reverse trnF1F TGCCAGAAAC CAGATTTGAA CTGGTGACAC Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
rps4trnTtrnLtrnF Reverse trnF8F TCAGTCCTCT GCTCTACCAA CTGAGCTA Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnLtrnF Reverse f [trnF(GAA)R-f] ATTTGAACTG GTGACACGAG Taberlet et al. (1991) Vascular plants
trnLtrnF Reverse d [trnL(UAA)3exonR-d] GGGGATAGAG GGACTTGAAC Taberlet et al. (1991) Vascular plants
trnLtrnF Reverse b [trnL(UAA)5exonR-b] TCTACCGATT TCGCCATATC Taberlet et al. (1991) Vascular plants

matK

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
trnK intron (including matK) trnK [tRNA-Lys(UAA)exon1] CCGACTAGTT CCGGGTTCGA Demesure et al. (1995) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK aF ARWTTCATCC AAACCATTGA CAAGG Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
matK 410F TATCAATCTA TTCATTCYGT ATTTCCTTTT Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
matK 410R AAAAGGAAAT ACRGAATGAA TAGATTGATA Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK aR ATTGCACACG GCTTTCTCTA TGT Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK [tRNA-Lys(UAA)exon2] CAACGGTAGA GTACTCGGCT TTTA Demesure et al. (1995) Bryophytes (Leucobryum, Oguri et al. 2013)

rpl16 G2 intron

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rpl16 G2 intron Forward F71 GCTATGCTTAGTGTGTGACTCGTTG Jordan et al. (1996) Vascular plants, Bryophytes (Jordan et al. 1996, Olsson et al. 2009)
rpl16 G2 intron Forward F435 CTCAAAAAGGTAAAGAGCCCTGAGT Jordan et al. (1996) Vascular plants (Jordan et al. 1996)
rpl16 G2 intron Reverse rpl16R GTAATCCAAGCTGGTTCAAGTGC Olsson et al. (2009) Bryophytes (Olsson et al. 2009)
rpl16 G2 intron Reverse R1661 CGTACCCATATTTTTCCACCACGAC Jordan et al. (1996) Vascular plants (Jordan et al. 1996)
rpl16 G2 intron Reverse R434 CTCAGGGCTCTTTACCTTTTTGAGT Jordan et al. (1996) Vascular plants (Jordan et al. 1996)

trnM-trnV [trnM-trnV intergenic region including trnV intron]

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
trnM-trnV Forward trnMF GCGATACTCTAAACCACTGAG Werner et al. (2009) Bryophytes (Werner et al. 2009)
trnM-trnV Reverse trnVR TYGAACCGTAGACATTCTCGG Werner et al. (2009) Bryophytes (Werner et al. 2009)

psbA-trnH

nuclear

18S rRNA gene and ITS region

References

関連ページ


デジタル自然史博物館 / 広島大学 / 植物 / コケ植物(蘚苔類) / 情報 にもどる