Ohta Katoh Miyake 1977
Ohta et al. (1977)
- Ohta, Y., Katoh, K. & Miyake, K. 1977. Establishment and growth characteristics of a cell suspension culture of Marchantia polymorpha L. with high chlorophyll content. Planta 136(3): 229-232. doi: 10.1007/BF00385989.
A green-pigmented cell suspension culture of Marchantia polymorpha was established using the medium of Murashige and Skoog with addition of organic acids of the tricarboxylic-acid cycle, vitamins and sugars plus sugar alcohols, exclusion of kinetin, and replacement of sucrose with glucose. In continuous light, the cells grew exponentially for ca. 10 days; in the dark, they grew only to a slight extent. The light-grown cells contained well-developed chloroplasts, and chlorophyll content reached almost twice that of the intact gametophyte.