Amplification fragment length polymorphism

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目次

amplification fragment length polymorphism (AFLP)

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Definition

Glossary of "Plant Systematics (1st ed.)" by Simpson (2006)

  • The use of amplified DNA fragments that exhibit length polymorphism, enabling the recognition of numerous genetic markers.

Glossary of "Genetic Management of Fragmented Animal and Plant Populations" by Frankham et al. (2017)

  • Method for detecting genetic variation across the genome by cutting genomic DNA with an enzyme that cleaves particular DNA sequences, adding short synthetic adapter DNA fragments of known sequence to the cut ends, carrying out PCR using primers that complement the adapter sequence, running amplified DNA on a gel, and visualizing the fragments. A multilocus DNA fingerprint is produced.

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