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提供: 広島大学デジタル博物館
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== Abstract ==
== Abstract ==
Tsubota, H., Inoue, Y., Nakahara-Tsubota, M., Shimamoto, T., Matsuda, I., Uchida, S. & Mukai, S. 2014. Combination of DNA barcoding and molecular phylogenetic analysis as an identification tool for plant species: a case study using imperfect herbarium specimens. Hikobia 16: 475-490.
Tsubota, H., Inoue, Y., Nakahara-Tsubota, M., Shimamoto, T., Matsuda, I., Uchida, S. & Mukai, S. 2014. Combination of DNA barcoding and molecular phylogenetic analysis as an identification tool for plant species: a case study using imperfect herbarium specimens. Hikobia 16: 475-490.
Some imperfect specimens deposited in the herbarium HIRO, which were difficult to identify to species level based on morphology, were identified using DNA barcoding combined with molecular phylogenetic analyses. Based on sequences of the chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (cp ''rbc''L) gene and the nuclear ribosomal internal transcribed spacer (nr ITS) region, ''Ludwigia ovalis'' Miq. (Onagraceae) and ''Juncus wallichianus'' Laharpe (Juncaceae) were identified from herbarium specimens collected in Miyajima Island, Hiroshima Prefecture, Honshu, southwestern Japan. ''L. ovalis'' was formerly regarded as extinct on the island. According to locality information on the specimens, these species grew in a small artificial pond in a small valley in forest dominated by ''Abies firma'' Siebold & Zucc. and ''Symplocos glauca'' (Thunb.) Koidz. The results demonstrate that DNA barcoding combined with molecular phylogenetic analyses are useful tools for species level identification of imperfect specimens.
== Pdfs ==
== Pdfs ==
