坪田 井上 中原-坪田 島本 松田 内田 向井 2014

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目次

坪田ほか(2014a)

文献(出典)

  • 坪田博美・井上侑哉・中原-坪田美保・島本俊樹・松田伊代・内田慎治・向井誠二. 2014. 標本同定のツールとしてのDNAバーコーディングと分子系統解析-広島県宮島で採集された標本の例-. Hikobia 16: 475-490. [Tsubota, H., Inoue, Y., Nakahara-Tsubota, M., Shimamoto, T., Matsuda, I., Uchida, S. & Mukai, S. 2014. Combination of DNA barcoding and molecular phylogenetic analysis as an identification tool for plant species: a case study using imperfect herbarium specimens. Hikobia 16: 475-490.]

ノート

要旨

坪田博美・井上侑哉・中原-坪田美保・島本俊樹・松田伊代・内田慎治・向井誠二. 2014. 標本同定のツールとしてのDNAバーコーディングと分子系統解析-広島県宮島で採集された標本の例-. Hikobia 16: 475-490.

Abstract

Tsubota, H., Inoue, Y., Nakahara-Tsubota, M., Shimamoto, T., Matsuda, I., Uchida, S. & Mukai, S. 2014. Combination of DNA barcoding and molecular phylogenetic analysis as an identification tool for plant species: a case study using imperfect herbarium specimens. Hikobia 16: 475-490.

Some imperfect specimens deposited in the herbarium HIRO, which were difficult to identify to species level based on morphology, were identified using DNA barcoding combined with molecular phylogenetic analyses. Based on sequences of the chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (cp rbcL) gene and the nuclear ribosomal internal transcribed spacer (nr ITS) region, Ludwigia ovalis Miq. (Onagraceae) and Juncus wallichianus Laharpe (Juncaceae) were identified from herbarium specimens collected in Miyajima Island, Hiroshima Prefecture, Honshu, southwestern Japan. L. ovalis was formerly regarded as extinct on the island. According to locality information on the specimens, these species grew in a small artificial pond in a small valley in forest dominated by Abies firma Siebold & Zucc. and Symplocos glauca (Thunb.) Koidz. The results demonstrate that DNA barcoding combined with molecular phylogenetic analyses are useful tools for species level identification of imperfect specimens.

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広島大学 / デジタル自然史博物館 / 植物 にもどる