PCR primers nr 18SrRNA ITSs

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広島大学 > デジタル自然史博物館 > 植物 > コケ植物(蘚苔類) > 情報

目次

PCR primers

nuclear

18S rRNA gene and ITS region

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
18S rRNA gene Forward 18SPrimer A CCGAATTCGT CGACAACCTG GTTGATCCTG CCAGT Medlin et al. (1988) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S1Fh AACCTGGTTG ATCCTGCC Nakahara et al. (2004) Algae, Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S2Fh GTTGATCCTG CCAGTAGTCA Nakahara et al. (2004) Algae
18S rRNA gene Forward 18SNS1 GTAGTCATAT GCTTGTCTC White et al. (1990); Kocher & White (1992) Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S323 CGATGGTAGG ATAGAGG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S410 CCACATCCAA GGAAGGCAGC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S575 CGGTAATTCC AGCTCCA Handa et al. (2003 Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S705 GGCTCCTGGG CTTCACTG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S921 GAAAGACGAA CTACTGCGA Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S1171 GCTTAATTTG ACTCAACAC Tsubota et al. (2012) Algae, Vascular plants (Lactuca etc.)
18S rRNA gene Forward 18S1208 TACCAGGTCC AGACATAGTG AGG Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Forward 18S1288 TGGGTTGCCT TGTCA Nakahara et al. (2004) Algae
18S rRNA gene Forward 18S1307 ACGAGACCTC AGCCTGCT Nakahara et al. (2004) Algae
18S rRNA gene Forward 18S1421 CAGGTCTGTG ATGCCCTTAG A Nakahara et al. (2004) Algae, Vascular plants (Lactuca etc.)
ITS Forward 18S1659B CGTCGCTCCT ACCGATTG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca etc.)
ITS Forward 18S1764B AGAGGAAGGA GAAGTCGTAA C Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca etc.)
ITS Forward 18SrDNAHTF1 AAGGTTTCCG TAGGTGAACC TGC Tsubota et al. (2012) Vascular plants (Lactuca etc.)
ITS Forward 18srRNA TCCGTAGGTG AACCTGCGG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Forward 5.8S10B CTCAGCAACG GATATCTTGG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Forward 5srRNA ACGATGAAGA ACGCAGCG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Forward 5.8S43H GAAGAACGTA GCGAAATGCG A Tsubota et al. (2009) Halophila Hikobia Vascular plants (Halophila, Lactuca etc.)
ITS Forward 5.8S51HV
ITS Forward 5.8S114HV
ITS Reverse 26S166BR GAGGACGCTT CTCCAGACTA C Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca etc.)
ITS Reverse 26S102BR CCGGTTCGCT CGCCG Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca etc.)
ITS Reverse 25srRNA TCCTCCGCTT AGTGATATGC Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Reverse 26SrDNAHTR1 Tsubota et al. (2012) Vascular plants (Lactuca etc.)
ITS Reverse 26SrDNAHTR2 AAGTTTCTTT TCCTCCGCTT A Tsubota et al. (2012) Vascular plants (Lactuca etc.)
ITS Reverse 5.8S124HR CGGGCGCAAC TTGCGTTCA Tsuota et al. (2009) Vascular plants (Halophila, Lactuca etc.)
ITS Reverse 5.8S102BR ACTCGATGAT TCGCGGA Oguri et al. (2003) Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
ITS Reverse 5srRNAR CGCTGCGTTC TTCATCGT Handa et al. (2003); Oguri et al. (2003) Algae (Stichococcus etc.), Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.)
18S rRNA gene & ITS Reverse 5.8SR-1 Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene & ITS Reverse 5srRNAR CGCTGCGTTC TTCATCGT Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Reverse 18Sprimer B CCCGGGATCC AAGCTTGATC CTTCTGCAGG TTCACCTAC Medlin et al. (1988) Algae (Stichococcus etc.)
18S rRNA gene Reverse 18S3Rh TGATCCTTCT GCAGGTTC Nakahara et al. (2004) Algae, Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18S4Rh CTGCAGGTTC ACCTACGGA Nakahara et al. (2004) Algae
18S rRNA gene Reverse 18S1617R CAGGGACGTA ATCAACGC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18S1442R TCTAAGGGCA TCACAGAC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18S1125R CAGCCTTGCG ACCATACTCC Handa et al. (2003) Algae (Stichococcus etc.)
18S rRNA gene Reverse 18S909R CCAAGAATTT CACCTCTGAC Handa et al. (2003) Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18SNS2 GGCTGCTGGC ACCAGACTTG C White et al. (1990); Kocher & White (1992) Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca etc.)
18S rRNA gene Reverse 18S325R++ CGAAAGTTGA TAGGGCAG Tsubota et al. (2012) Algae, Vascular plants (Lactuca etc.)

References

  • Handa, S., Nakahara, M., Tsubota, H., Deguchi, H. & Nakano, T. 2003. A new aerial alga, Stichococcus ampulliformis sp. nov. (Trebouxiophyceae, Chlorophyta) from Japan. Phycol. Res. 51: 203-210.
  • Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17: 1105-1109.
  • Tsubota, H., Kubo, H. & Mukai, S. 2009. A new finding of seagrass Halophila nipponica J.Kuo (Hydrocharitaceae) from Miyajima Island in Hiroshima Prefecture, SW Japan. Hikobia 15: 339-347 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・向井誠二. 2009. 広島県宮島近海で見つかったヤマトウミヒルモHalophila nipponica J.Kuoについて. Hikobia 15: 339-347.]
  • Tsubota, H., Kubo, H., Takeuchi, K., Nakahara-Tsubota, M., Inoue, Y., Uchida, S. & Mukai, S. 2012. Notes on naturalized plants in Hiroshima Prefecture: 3. New locality and nrITS variation of Lactuca serriola L. (Asteraceae). Hikobia 16: 197-202 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・武内一恵・中原-坪田美保・井上侑哉・内田慎治・向井誠二. 2012. 広島の帰化植物3.トゲヂシャとマルバトゲヂシャ. Hikobia 16: 197-202.]

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