「PCR primers nr 18SrRNA ITSs」の版間の差分
提供: 広島大学デジタル博物館
ナビゲーションに移動検索に移動| 26行目: | 26行目: | ||
| <tt>AACCTGGTTG ATCCTGCC</tt> | | <tt>AACCTGGTTG ATCCTGCC</tt> | ||
| Nakahara et al. (2004) | | Nakahara et al. (2004) | ||
| − | | Algae, Vascular plants (''Lactuca'' etc.) | + | | Algae, Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 54行目: | 54行目: | ||
| <tt>CCACATCCAA GGAAGGCAGC</tt> | | <tt>CCACATCCAA GGAAGGCAGC</tt> | ||
| Handa et al. (2003) | | Handa et al. (2003) | ||
| − | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'' etc.) | + | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 75行目: | 75行目: | ||
| <tt>GAAAGACGAA CTACTGCGA</tt> | | <tt>GAAAGACGAA CTACTGCGA</tt> | ||
| Handa et al. (2003) | | Handa et al. (2003) | ||
| − | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'' etc.) | + | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 110行目: | 110行目: | ||
| <tt>CAGGTCTGTG ATGCCCTTAG A</tt> | | <tt>CAGGTCTGTG ATGCCCTTAG A</tt> | ||
| Nakahara et al. (2004) | | Nakahara et al. (2004) | ||
| − | | Algae, Vascular plants (''Lactuca'' etc.) | + | | Algae, Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 117行目: | 117行目: | ||
| <tt>CGTCGCTCCT ACCGATTG</tt> | | <tt>CGTCGCTCCT ACCGATTG</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 124行目: | 124行目: | ||
| <tt>AGAGGAAGGA GAAGTCGTAA C</tt> | | <tt>AGAGGAAGGA GAAGTCGTAA C</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 131行目: | 131行目: | ||
| <tt>AAGGTTTCCG TAGGTGAACC TGC</tt> | | <tt>AAGGTTTCCG TAGGTGAACC TGC</tt> | ||
| Tsubota et al. (2012) | | Tsubota et al. (2012) | ||
| − | | Vascular plants (''Lactuca'' etc.) | + | | Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 138行目: | 138行目: | ||
| <tt>TCCGTAGGTG AACCTGCGG</tt> | | <tt>TCCGTAGGTG AACCTGCGG</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 159行目: | 159行目: | ||
| <tt>GAAGAACGTA GCGAAATGCG A</tt> | | <tt>GAAGAACGTA GCGAAATGCG A</tt> | ||
| Tsubota et al. (2009) Halophila Hikobia | | Tsubota et al. (2009) Halophila Hikobia | ||
| − | | Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 187行目: | 187行目: | ||
| <tt>GAGGACGCTT CTCCAGACTA C</tt> | | <tt>GAGGACGCTT CTCCAGACTA C</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 194行目: | 194行目: | ||
| <tt>CCGGTTCGCT CGCCG</tt> | | <tt>CCGGTTCGCT CGCCG</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 201行目: | 201行目: | ||
| <tt>TCCTCCGCTT AGTGATATGC</tt> | | <tt>TCCTCCGCTT AGTGATATGC</tt> | ||
| Oguri et al. (2003) | | Oguri et al. (2003) | ||
| − | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'' etc.) | + | | Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 208行目: | 208行目: | ||
| <tt>AAGGGAATCC TTGTAAGTTT CT</tt> | | <tt>AAGGGAATCC TTGTAAGTTT CT</tt> | ||
| Tsubota et al. (2012) | | Tsubota et al. (2012) | ||
| − | | Vascular plants (''Lactuca'' etc.) | + | | Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 215行目: | 215行目: | ||
| <tt>AAGTTTCTTT TCCTCCGCTT A</tt> | | <tt>AAGTTTCTTT TCCTCCGCTT A</tt> | ||
| Tsubota et al. (2012) | | Tsubota et al. (2012) | ||
| − | | Vascular plants (''Lactuca'' etc.) | + | | Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 222行目: | 222行目: | ||
| <tt>CGGGCGCAAC TTGCGTTCA</tt> | | <tt>CGGGCGCAAC TTGCGTTCA</tt> | ||
| Tsuota et al. (2009) | | Tsuota et al. (2009) | ||
| − | | Vascular plants (''Halophila'', ''Lactuca'' etc.) | + | | Vascular plants (''Halophila'', ''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| ITS | | ITS | ||
| 236行目: | 236行目: | ||
| <tt>CGCTGCGTTC TTCATCGT</tt> | | <tt>CGCTGCGTTC TTCATCGT</tt> | ||
| Handa et al. (2003); Oguri et al. (2003) | | Handa et al. (2003); Oguri et al. (2003) | ||
| − | | Algae (''Stichococcus'' etc.), Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'' etc.) | + | | Algae (''Stichococcus'' etc.), Bryophytes (''Leucobryum'' etc.), Vascular plants (''Halophila'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene & ITS | | 18S rRNA gene & ITS | ||
| 264行目: | 264行目: | ||
| <tt>TGATCCTTCT GCAGGTTC</tt> | | <tt>TGATCCTTCT GCAGGTTC</tt> | ||
| Nakahara et al. (2004) | | Nakahara et al. (2004) | ||
| − | | Algae, Vascular plants (''Lactuca'' etc.) | + | | Algae, Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 285行目: | 285行目: | ||
| <tt>TCTAAGGGCA TCACAGAC</tt> | | <tt>TCTAAGGGCA TCACAGAC</tt> | ||
| Handa et al. (2003) | | Handa et al. (2003) | ||
| − | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'' etc.) | + | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 299行目: | 299行目: | ||
| <tt>CCAAGAATTT CACCTCTGAC</tt> | | <tt>CCAAGAATTT CACCTCTGAC</tt> | ||
| Handa et al. (2003) | | Handa et al. (2003) | ||
| − | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'' etc.) | + | | Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
| 306行目: | 306行目: | ||
| <tt>GGCTGCTGGC ACCAGACTTG C</tt> | | <tt>GGCTGCTGGC ACCAGACTTG C</tt> | ||
| White et al. (1990); Kocher & White (1992) | | White et al. (1990); Kocher & White (1992) | ||
| − | | Fungi, Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'' etc.) | + | | Fungi, Algae (''Stichococcus'' etc.), Vascular plants (''Lactuca'', ''Sisymbrium'' etc.) |
|- | |- | ||
| 18S rRNA gene | | 18S rRNA gene | ||
2013年12月31日 (火) 19:53時点における版
広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報
PCR primers
nuclear
18S rRNA gene and ITS region
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| 18S rRNA gene | Forward | 18SPrimer A | CCGAATTCGT CGACAACCTG GTTGATCCTG CCAGT | Medlin et al. (1988) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Forward | 18S1Fh | AACCTGGTTG ATCCTGCC | Nakahara et al. (2004) | Algae, Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Forward | 18S2Fh | GTTGATCCTG CCAGTAGTCA | Nakahara et al. (2004) | Algae |
| 18S rRNA gene | Forward | 18SNS1 | GTAGTCATAT GCTTGTCTC | White et al. (1990); Kocher & White (1992) | Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca etc.) |
| 18S rRNA gene | Forward | 18S323 | CGATGGTAGG ATAGAGG | Handa et al. (2003) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Forward | 18S410 | CCACATCCAA GGAAGGCAGC | Handa et al. (2003) | Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Forward | 18S575 | CGGTAATTCC AGCTCCA | Handa et al. (2003 | Algae (Stichococcus etc.), Vascular plants (Lactuca etc.) |
| 18S rRNA gene | Forward | 18S705 | GGCTCCTGGG CTTCACTG | Handa et al. (2003) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Forward | 18S921 | GAAAGACGAA CTACTGCGA | Handa et al. (2003) | Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Forward | 18S1171 | GCTTAATTTG ACTCAACAC | Tsubota et al. (2012) | Algae, Vascular plants (Lactuca etc.) |
| 18S rRNA gene | Forward | 18S1208 | TACCAGGTCC AGACATAGTG AGG | Handa et al. (2003) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Forward | 18S1288 | TGGGTTGCCT TGTCA | Nakahara et al. (2004) | Algae |
| 18S rRNA gene | Forward | 18S1307 | ACGAGACCTC AGCCTGCT | Nakahara et al. (2004) | Algae |
| 18S rRNA gene | Forward | 18S1421 | CAGGTCTGTG ATGCCCTTAG A | Nakahara et al. (2004) | Algae, Vascular plants (Lactuca, Sisymbrium etc.) |
| ITS | Forward | 18S1659B | CGTCGCTCCT ACCGATTG | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Forward | 18S1764B | AGAGGAAGGA GAAGTCGTAA C | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Forward | 18SrDNAHTF1 | AAGGTTTCCG TAGGTGAACC TGC | Tsubota et al. (2012) | Vascular plants (Lactuca, Sisymbrium etc.) |
| ITS | Forward | 18srRNA | TCCGTAGGTG AACCTGCGG | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.) |
| ITS | Forward | 5.8S10B | CTCAGCAACG GATATCTTGG | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.) |
| ITS | Forward | 5srRNA | ACGATGAAGA ACGCAGCG | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.) |
| ITS | Forward | 5.8S43H | GAAGAACGTA GCGAAATGCG A | Tsubota et al. (2009) Halophila Hikobia | Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Forward | 5.8S51HV | TAGCGAAATG CGATACTTGG | Tsubota et al. (2013) Sisymbrium Hikobia | Vascular plants (Sisymbrium etc.) |
| ITS | Forward | 5.8S114HV | AGTTGCGCCC GAAGC | Tsubota et al. (2013) Sisymbrium Hikobia | Vascular plants (Sisymbrium etc.) |
| ITS | Reverse | 26S166BR | GAGGACGCTT CTCCAGACTA C | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Reverse | 26S102BR | CCGGTTCGCT CGCCG | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Reverse | 25srRNA | TCCTCCGCTT AGTGATATGC | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.) |
| ITS | Reverse | 26SrDNAHTR1 | AAGGGAATCC TTGTAAGTTT CT | Tsubota et al. (2012) | Vascular plants (Lactuca, Sisymbrium etc.) |
| ITS | Reverse | 26SrDNAHTR2 | AAGTTTCTTT TCCTCCGCTT A | Tsubota et al. (2012) | Vascular plants (Lactuca, Sisymbrium etc.) |
| ITS | Reverse | 5.8S124HR | CGGGCGCAAC TTGCGTTCA | Tsuota et al. (2009) | Vascular plants (Halophila, Lactuca, Sisymbrium etc.) |
| ITS | Reverse | 5.8S102BR | ACTCGATGAT TCGCGGA | Oguri et al. (2003) | Bryophytes (Leucobryum etc.), Vascular plants (Halophila etc.) |
| ITS | Reverse | 5srRNAR | CGCTGCGTTC TTCATCGT | Handa et al. (2003); Oguri et al. (2003) | Algae (Stichococcus etc.), Bryophytes (Leucobryum etc.), Vascular plants (Halophila, Sisymbrium etc.) |
| 18S rRNA gene & ITS | Reverse | 5.8SR-1 | Handa et al. (2003) | Algae (Stichococcus etc.) | |
| 18S rRNA gene & ITS | Reverse | 5srRNAR | CGCTGCGTTC TTCATCGT | Handa et al. (2003) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Reverse | 18Sprimer B | CCCGGGATCC AAGCTTGATC CTTCTGCAGG TTCACCTAC | Medlin et al. (1988) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Reverse | 18S3Rh | TGATCCTTCT GCAGGTTC | Nakahara et al. (2004) | Algae, Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Reverse | 18S4Rh | CTGCAGGTTC ACCTACGGA | Nakahara et al. (2004) | Algae |
| 18S rRNA gene | Reverse | 18S1617R | CAGGGACGTA ATCAACGC | Handa et al. (2003) | Algae (Stichococcus etc.), Vascular plants (Lactuca etc.) |
| 18S rRNA gene | Reverse | 18S1442R | TCTAAGGGCA TCACAGAC | Handa et al. (2003) | Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Reverse | 18S1125R | CAGCCTTGCG ACCATACTCC | Handa et al. (2003) | Algae (Stichococcus etc.) |
| 18S rRNA gene | Reverse | 18S909R | CCAAGAATTT CACCTCTGAC | Handa et al. (2003) | Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Reverse | 18SNS2 | GGCTGCTGGC ACCAGACTTG C | White et al. (1990); Kocher & White (1992) | Fungi, Algae (Stichococcus etc.), Vascular plants (Lactuca, Sisymbrium etc.) |
| 18S rRNA gene | Reverse | 18S325R++ | CGAAAGTTGA TAGGGCAG | Tsubota et al. (2012) | Algae, Vascular plants (Lactuca etc.) |
References
- Handa, S., Nakahara, M., Tsubota, H., Deguchi, H. & Nakano, T. 2003. A new aerial alga, Stichococcus ampulliformis sp. nov. (Trebouxiophyceae, Chlorophyta) from Japan. Phycol. Res. 51: 203-210.
- Tsubota, H., Kubo, H. & Mukai, S. 2009. A new finding of seagrass Halophila nipponica J.Kuo (Hydrocharitaceae) from Miyajima Island in Hiroshima Prefecture, SW Japan. Hikobia 15: 339-347 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・向井誠二. 2009. 広島県宮島近海で見つかったヤマトウミヒルモHalophila nipponica J.Kuoについて. Hikobia 15: 339-347.]
- Tsubota, H., Kubo, H., Takeuchi, K., Nakahara-Tsubota, M., Inoue, Y., Uchida, S. & Mukai, S. 2012. Notes on naturalized plants in Hiroshima Prefecture: 3. New locality and nrITS variation of Lactuca serriola L. (Asteraceae). Hikobia 16: 197-202 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・武内一恵・中原-坪田美保・井上侑哉・内田慎治・向井誠二. 2012. 広島の帰化植物3.トゲヂシャとマルバトゲヂシャ. Hikobia 16: 197-202.]
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