Amplification fragment length polymorphism
提供: 広島大学デジタル博物館
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amplification fragment length polymorphism (AFLP)
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Definition
Glossary of "Plant Systematics (1st ed.)" by Simpson (2006)
- The use of amplified DNA fragments that exhibit length polymorphism, enabling the recognition of numerous genetic markers.
Glossary of "Genetic Management of Fragmented Animal and Plant Populations" by Frankham et al. (2017)
- Method for detecting genetic variation across the genome by cutting genomic DNA with an enzyme that cleaves particular DNA sequences, adding short synthetic adapter DNA fragments of known sequence to the cut ends, carrying out PCR using primers that complement the adapter sequence, running amplified DNA on a gel, and visualizing the fragments. A multilocus DNA fingerprint is produced.
文献（引用）
- Frankham, R., Ballou, J. D., Ralls, K., Eldridge, M. D. B., Dudash, M. R., Fenster, C. B., Lacy, R. C. & Sunnucks, P. 2017. Genetic Management of Fragmented Animal and Plant Populations. -xxiii + 401 pp. Oxford University Press, Oxford.
- Simpson, M. G. 2006. Plant Systematics. -xi + 590 pp. Elsevier Academic Press, Oxford.
